Journal: Tissue Engineering Part A

Article Title: Human Adipose Tissue-Derived SSEA-4 Subpopulation Multi-Differentiation Potential Towards the Endothelial and Osteogenic Lineages

doi: 10.1089/ten.tea.2012.0092

Figure Lengend Snippet: FIG. 2. In vitro osteogenic differentiation of hASCs and SSEA-4+hASCs. (A) quantitative RT-polymerase chain reaction (qRT-PCR) results showing the overexpression of osteogenic- related genes, osteocalcin (OCN), and osteopontin (OPN) in both cell populations along the culture in osteogenic conditions (B, C) Immunostaining of OPN and OCN deposition by hASCs (B) and SSEA-4+

Article Snippet: Cells were incubated for 1 h at room temperature with the primary antibodies mouse anti-human CD31 (1:50; Dako), rabbit anti-human von Willebrand factor (vWF, 1:200; Dako), mouse anti-human SSEA-4 (1:50; Abcam), CD34-PE (1:50; BD Biosciences), and mouse antihuman CD105 (1:50; eBioscience), mouse anti-human osteocalcin (OCN, 1:50, AbD Serotec), and rabbit anti-human osteopontin (OPN, 1:50; Abcam).

Techniques: In Vitro, Polymerase Chain Reaction, Quantitative RT-PCR, Over Expression, Immunostaining

Journal: Polymers

Article Title: Simple Electrospinning Method for Biocompatible Polycaprolactone β-Carotene Scaffolds: Advantages and Limitations

doi: 10.3390/polym16101371

Figure Lengend Snippet: Biological testing results with KUSA-A1 and E. coli : ( a ) KUSA-A1 cell optical density, ( b ) area coverage of the osteocalcin staining with respect to the investigated area of the sample, ( c ) E. coli optical density. Results marked with “*” have a p -value < 0.05 when compared with all others.

Article Snippet: Osteogenic differentiation was assessed after 10 days in the osteogenic medium by staining cultured KUSA-A1 cells with the green osteocalcin stain (Osteocalcin Mouse anti-Human, PE, Clone, Fisher Scientific, Hampton, NH, USA) following the manufacturer’s instructions.

Techniques: Staining